Detection of a CpA methylase in an insect system: characterization and substrate specificity


Autoria(s): Devajyothi, Chalasani; Brahmachari, Vani
Data(s)

25/03/1992

Resumo

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependance similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/35166/1/Detection_of_a_CpA.pdf

Devajyothi, Chalasani and Brahmachari, Vani (1992) Detection of a CpA methylase in an insect system: characterization and substrate specificity. In: Molecular and Cellular Biochemistry, 110 (2). pp. 103-111.

Publicador

Springer

Relação

http://www.springerlink.com/content/287705734755790u/

http://eprints.iisc.ernet.in/35166/

Palavras-Chave #Microbiology & Cell Biology
Tipo

Journal Article

PeerReviewed