Dual-trap optical tweezers with real-time force clamp control


Autoria(s): Wallin, Anders; Ojala, Heikki Viljami; Ziedaite, Gabija; Haeggström, Edward
Contribuinte(s)

University of Helsinki, Department of Physics

University of Helsinki, Department of Physics

University of Helsinki, Department of Biosciences

University of Helsinki, Department of Physics

Data(s)

03/08/2011

Resumo

Single molecule force clamp experiments are widely used to investigate how enzymes, molecular motors, and other molecular mechanisms work. We developed a dual-trap optical tweezers instrument with real-time (200 kHz update rate) force clamp control that can exert 0–100 pN forces on trapped beads. A model for force clamp experiments in the dumbbell-geometry is presented. We observe good agreement between predicted and observed power spectra of bead position and force fluctuations. The model can be used to predict and optimize the dynamics of real-time force clamp optical tweezers instruments. The results from a proof-of-principle experiment in which lambda exonuclease converts a double-stranded DNA tether, held at constant tension, into its single-stranded form, show that the developed instrument is suitable for experiments in single molecule biology.

Formato

7

Identificador

http://hdl.handle.net/10138/27789

0034-6748

Idioma(s)

eng

Relação

Review of Scientific Instruments

Fonte

Wallin , A , Ojala , H V , Ziedaite , G & Haeggström , E 2011 , ' Dual-trap optical tweezers with real-time force clamp control ' Review of Scientific Instruments , vol 82 , no. 8 , pp. 083102 . , 10.1063/1.3615309

Palavras-Chave #114 Physical sciences
Tipo

A1 Refereed journal article

info:eu-repo/semantics/article

info:eu-repo/semantics/acceptedVersion

info:eu-repo/semantics/acceptedVersion