Rapid isolation of lysyl-tRNA synthetase from rat liver
| Data(s) |
29/04/1988
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|---|---|
| Resumo |
The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 6.1.1.6) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield. |
| Formato |
application/pdf |
| Identificador |
http://eprints.iisc.ernet.in/32526/1/RAPID_ISOLATION.pdf Kumar, Madan A and Nayak, R (1988) Rapid isolation of lysyl-tRNA synthetase from rat liver. In: Biochemical and Biophysical Research Communications, 152 (2). 593 -597. |
| Publicador |
Elsevier science |
| Relação |
http://dx.doi.org/10.1016/S0006-291X(88)80079-2 http://eprints.iisc.ernet.in/32526/ |
| Palavras-Chave | #Microbiology & Cell Biology |
| Tipo |
Journal Article PeerReviewed |
