Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA
Data(s) |
15/07/1988
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Resumo |
Antibodies raised against deoxyadenylate and deoxycytidylate were found to react with double stranded DNA as assessed by highly sensitive avidin-biotin microELISA. The binding was specific as it was completely inhibited by the homologous hapten. The antibodies did not react with tRNA and rRNA. These antibodies were also shown to react with supercoiled and relaxed forms of pBR322 DNA as demonstrated by gel retardation assay. ssDNA, single-stranded DNA; dsDNA, double-stranded DNA; CT DNA, calf thymus DNA; AB microELISA, avidin-biotin microELISA; dpA, deoxyadenylate; dpC, deoxycytidylate; avidin-HRP, avidin-horseradish peroxidase |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/32050/1/binding.pdf Vaishnav, YN and Antony, A (1988) Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA. In: Biochemical and Biophysical Research Communications, 154 (1). pp. 118-123. |
Publicador |
Elsevier science |
Relação |
http://dx.doi.org/10.1016/0006-291X(88)90658-4 http://eprints.iisc.ernet.in/32050/ |
Palavras-Chave | #Microbiology & Cell Biology |
Tipo |
Journal Article PeerReviewed |