Unusual RNA plant virus integration in the soybean genome leads to the production of small RNAs


Autoria(s): Cordenonsi da Fonseca, Guilherme; Valter de Oliveira, Luis Felipe; Loss de Morais, Guilherme; Abdelnor, Ricardo Vilela; Nepomuceno, Alexandre Lima; Waterhouse, Peter M.; Farinelli, Laurent; Margis, Rogerio
Data(s)

01/05/2016

Resumo

Horizontal gene transfer (HGT) is known to be a major force in genome evolution. The acquisition of genes from viruses by eukaryotic genomes is a well-studied example of HGT, including rare cases of non-retroviral RNA virus integration. The present study describes the integration of cucumber mosaic virus RNA-1 into soybean genome. After an initial metatranscriptomic analysis of small RNAs derived from soybean, the de novo assembly resulted a 3029-nt contig homologous to RNA-1. The integration of this sequence in the soybean genome was confirmed by DNA deep sequencing. The locus where the integration occurred harbors the full RNA-1 sequence followed by the partial sequence of an endogenous mRNA and another sequence of RNA-1 as an inverted repeat and allowing the formation of a hairpin structure. This region recombined into a retrotransposon located inside an exon of a soybean gene. The nucleotide similarity of the integrated sequence compared to other Cucumber mosaic virus sequences indicates that the integration event occurred recently. We described a rare event of non-retroviral RNA virus integration in soybean that leads to the production of a double-stranded RNA in a similar fashion to virus resistance RNAi plants.

Identificador

http://eprints.qut.edu.au/95500/

Publicador

Elsevier Ireland Ltd.

Relação

DOI:10.1016/j.plantsci.2016.01.011

Cordenonsi da Fonseca, Guilherme, Valter de Oliveira, Luis Felipe, Loss de Morais, Guilherme, Abdelnor, Ricardo Vilela, Nepomuceno, Alexandre Lima, Waterhouse, Peter M., Farinelli, Laurent, & Margis, Rogerio (2016) Unusual RNA plant virus integration in the soybean genome leads to the production of small RNAs. Plant Science, 246, pp. 62-69.

Direitos

Copyright 2016 Elsevier Ireland Ltd.

Fonte

Centre for Tropical Crops and Biocommodities; School of Earth, Environmental & Biological Sciences; Institute for Future Environments; Science & Engineering Faculty

Palavras-Chave #Horizontal gene transfer #RNA plant virus #Cucumber mosaic virus #Soybean #Transposon
Tipo

Journal Article