Metal-ion-mediated tuning of duplex DNA binding by bis(2-(2-pyridyl)-1H-benzimidazole)
Data(s) |
2007
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Resumo |
Studies of double-stranded-DNA binding have been performed with three isomeric bis)2-(n-pyridyl)-1H-benzimidazole)s (n = 2, 3, 4). Like the well-known Hoechst 33258, which is a bisbenzimidazole compound, these three isomers bind to the minor groove of duplex DNA. DNA binding by the three isomers was investigated in the presence of the divalent metal ions Mg2+, Co2+, Ni2+, Cu2+, and Zn2+. Ligand-DNA interactions were probed with fluorscence and circular dichroism spectroscopy. These studies revealed that the binding of the 2-pyridyl derivative to DNA is dramatically reduced in the presence of Co2+, Ni2+, and Cu2+ ions and is abolished completely at a ligand/metal-cation ratio of 1:1. Control experiments done with the isomeric 3- and 4-pyridyl derivatives showed that their binding to DNA is unaffected by the aforementioned transition-metal ions. The ability of 2-(2-pyridyl)benzimidazole changes of the ligand associated with ion chelation probably ledto such unusual binding results for the ortho isomer. The addition of ethylenediaminetetraacetic acid (EDTA) reversed the effects completely. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/27088/1/ya.pdf Bhattacharya, Santanu and Chaudhuri, Padmaparna (2007) Metal-ion-mediated tuning of duplex DNA binding by bis(2-(2-pyridyl)-1H-benzimidazole). In: Chemistry - An Asian Journal, 2 (5). pp. 648-655. |
Publicador |
John Wiley and Sons |
Relação |
http://www3.interscience.wiley.com/journal/114215676/abstract http://eprints.iisc.ernet.in/27088/ |
Palavras-Chave | #Organic Chemistry |
Tipo |
Journal Article PeerReviewed |