Evolution of sequence specificity in a restriction endonuclease by a point mutation


Autoria(s): Saravanan, Matheshwaran; Vasu, Kommireddy; Nagaraja, Valakunja
Data(s)

29/07/2008

Resumo

Restriction endonucleases (REases) protect bacteria from invading foreign DNAs and are endowed with exquisite sequence specificity. REases have originated from the ancestral proteins and evolved new sequence specificities by genetic recombination, gene duplication, replication slippage, and transpositional events. They are also speculated to have evolved from nonspecific endonucleases, attaining a high degree of sequence specificity through point mutations. We describe here an example of generation of exquisitely site-specific REase from a highly-promiscuous one by a single point mutation.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/26294/1/10344.full.pdf

Saravanan, Matheshwaran and Vasu, Kommireddy and Nagaraja, Valakunja (2008) Evolution of sequence specificity in a restriction endonuclease by a point mutation. In: PNAS, 105 (30). pp. 10344-10347.

Publicador

National Academy of Sciences

Relação

http://www.pnas.org/content/105/30/10344.full

http://eprints.iisc.ernet.in/26294/

Palavras-Chave #Microbiology & Cell Biology
Tipo

Journal Article

PeerReviewed