Mammalian brain plasma membranes: Isolation, enzymatic, and chemical characterization


Autoria(s): Krishnan, KS; Balaram, P
Data(s)

01/09/1976

Resumo

Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains, are described. The preparations show marked enrichments in the marker enzymes, (Na+-K+) adenosine triphosphatase, acetylcholinesterase, 5′-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern are presented. An enzymatic check has been made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins show a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.

Formato

application/pdf

Identificador

http://eprints.iisc.ernet.in/24041/1/article.pdf

Krishnan, KS and Balaram, P (1976) Mammalian brain plasma membranes: Isolation, enzymatic, and chemical characterization. In: Experimental Cell Research, 101 (2). pp. 299-306.

Publicador

Elservier Science

Relação

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFC-4F029RN-BG&_user=512776&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000025298&_version=1&_urlVersion=0&_userid=512776&md5=11369cc5b85ff64499d19d4760e81461

http://eprints.iisc.ernet.in/24041/

Palavras-Chave #Molecular Biophysics Unit
Tipo

Journal Article

PeerReviewed