Interactions of aromatic mannosyl disulfide derivatives with Concanavalin A: synthesis, thermodynamic and NMR spectroscopy studies
Data(s) |
08/09/2009
|
---|---|
Resumo |
α-d-Mannopyranosyl units were attached to an aromatic scaffold through disulfide linkages to obtain mono- to trivalent glycosylated ligands for lectin binding studies. Isothermal titration calorimetric (ITC) measurements indicated that binding affinities of these derivatives to Concanavalin A (Con A) were comparable to or slightly higher than that of methyl α-d-mannopyranoside (Ka values in the range of 104 M−1). The stoichiometries of the lectin-ligand complexes were in agreement with the formal valencies (1–3) of the respective ligands indicating cross-linking in interactions with the di- and trivalent derivatives. Multivalency effects could not, however, be observed with the latter. These ligands were shown to bind to the carbohydrate binding site of Con A using saturation transfer difference (STD) NMR competition experiments. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/23836/1/1111.pdf Murthy, Bandaru Narasimha and Sinha, Sharmistha and Surolia, Avadhesha and Jayaraman, Narayanaswamy and Szilagyi, Laszlo and Szabo, Ildiko and Kover, Katalin E (2009) Interactions of aromatic mannosyl disulfide derivatives with Concanavalin A: synthesis, thermodynamic and NMR spectroscopy studies. In: Carbohydrate Research, 344 (13). pp. 1758-1763. |
Publicador |
Elsevier Science |
Relação |
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFF-4WGF14S-5&_user=512776&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000025298&_version=1&_urlVersion=0&_userid=512776&md5=9decf5eefaf372c1f6d2c9e7a67d2a71 http://eprints.iisc.ernet.in/23836/ |
Palavras-Chave | #Organic Chemistry |
Tipo |
Journal Article PeerReviewed |