Enzymatic acylation of 1-alkyl-, 1-alkenyl-and 1-acyl glycero-3-phosphorylethanolamine in developing rat brain
Data(s) |
1974
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Resumo |
Rat brain particulate fractions were shown to acylate [32P]1-alkyl-sn-glycero-3-phosphorylethanolamine (GPE). While the main product is 1-alkyl-2-acyl GPE, about 12 per cent of the radioactivity was also found in 1-alkenyl-2-acyl GPE. The acyl transferase activity was completely dependent on added ATP and CoA and it was localized mainly in the microsomal fraction. A comparative study of acyl transferase activities to 1-alkyl-, 1-alkenyl-, and 1-acyl GPE by crude mitochondrial fraction and microsomes of 10, 16 and 22-day-old rat brains showed a progressive increase in activity with development. In the 22-day-old rat brain the order of activity towards the three substrates is as follows: 1-acyl GPE ± 1-alkenyl GPE ± 1-alkyl GPE with a crude mitochondrial fraction and 1-acyl GPE ± 1-alkyl GPE ± 1-alkenyl GPE with microsomes. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/23227/1/fulltext1.pdf Natarajan, V and Sastry, PS (1974) Enzymatic acylation of 1-alkyl-, 1-alkenyl-and 1-acyl glycero-3-phosphorylethanolamine in developing rat brain. In: Journal of Neurochemistry, 23 (1). pp. 187-192. |
Publicador |
John Wiley and Sons |
Relação |
http://www3.interscience.wiley.com/journal/119663450/abstract http://eprints.iisc.ernet.in/23227/ |
Palavras-Chave | #Biochemistry |
Tipo |
Journal Article PeerReviewed |