Separation of 5-methylcytosine-rich DNA using immobilized antibody
| Data(s) |
01/02/1986
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|---|---|
| Resumo |
Anti-(5-methylcytosine) antibodies were immobilized on glutaraldehyde-activated Indion 48-R, a polystyrene resin with amino groups. Immobilized antibody was very stable and could be used several times without any apparent change in the initial binding capacity of the antibody-matrix. Fractions of total DNA from various animal and plant sources were retained on this column and could be eluted quantitatively with 1.0 m NaCl. The bound fraction was further characterized for its 5-methylcytosine content by restriction enzyme digestion patterns. |
| Formato |
application/pdf |
| Identificador |
Deobagkar, DN and Shankar, V and Deobagkar, DD (1986) Separation of 5-methylcytosine-rich DNA using immobilized antibody. In: Enzyme and Microbial Technology, 8 (2). pp. 97-100. |
| Publicador |
Elsevier Science |
| Relação |
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG1-47GMHN9-K1&_user=512776&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000025298&_version=1&_urlVersion=0&_userid=512776&md5=9c148f957119b84c4485eabb9964d890 http://eprints.iisc.ernet.in/22725/ |
| Palavras-Chave | #Microbiology & Cell Biology |
| Tipo |
Journal Article PeerReviewed |
