Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers
Data(s) |
01/03/2010
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Resumo |
The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however, robust markers can be difficult and costly to isolate. To increase the number of available markers, dugong and manatee microsatellite primers were evaluated for cross-species amplification. Furthermore, one manatee and four dugong novel primers are reported. After polymerase chain reaction optimization, 23 (92%) manatee primers successfully amplified dugong DNA, of which 11 (48%) were polymorphic. Of the 32 dugong primers tested, 27 (84%) yielded product in the manatee, of which 17 (63%) were polymorphic. Dugong and manatee primers were compared and the most informative markers were selected to create robust and informative marker-panels for each species. These cross-species microsatellite marker-panels can be employed to assess other sirenian populations and can provide beneficial information for the protection and management of these unique mammals. |
Identificador |
Hunter, M. and Broderick, D. and Ovenden, J.R. and Tucker, K. and Bonde, R.K. and McGuire, P.M. and Lanyon, J. (2010) Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers. Molecular Ecology Resources, 10 (2). pp. 368-377. |
Publicador |
John Wiley & Sons |
Relação |
http://dx.doi.org/10.1111/j.1755-0998.2009.02761.x http://era.daf.qld.gov.au/1993/ |
Palavras-Chave | #Fisheries #Genetics #Chordates. Vertebrates |
Tipo |
Article PeerReviewed |