Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings
Data(s) |
15/08/1980
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Resumo |
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/21263/1/fulltext.pdf Srivenugopal, KS and Adiga, PR (1980) Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings. In: Bochemical Journal, 190 (2). pp. 461-464. |
Publicador |
The Biochemical Society |
Relação |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7470060 http://eprints.iisc.ernet.in/21263/ |
Palavras-Chave | #Biochemistry |
Tipo |
Journal Article PeerReviewed |