Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland
Data(s) |
2007
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Resumo |
Aims: To investigate the occurrence and levels of Arcobacter spp. in pig effluent ponds and effluent-treated soil. Methods and Results: A Most Probable Number (MPN) method was developed to assess the levels of Arcobacter spp. in seven pig effluent ponds and six effluent-treated soils, immediately after effluent irrigation. Arcobacter spp. levels in the effluent ponds varied from 6.5 × 105 to 1.1 × 108 MPN 100 ml-1 and in freshly irrigated soils from 9.5 × 102 to 2.8 × 104 MPN g-1 in all piggery environments tested. Eighty-three Arcobacter isolates were subjected to an abbreviated phenotypic test scheme and examined using a multiplex polymerase chain reaction (PCR). The PCR identified 35% of these isolates as Arcobacter butzleri, 49% as Arcobacter cryaerophilus while 16% gave no band. All 13 nonreactive isolates were subjected to partial 16S rDNA sequencing and showed a high similarity (>99%) to Arcobacter cibarius. Conclusions: A. butzleri, A. cryaerophilus and A. cibarius were isolated from both piggery effluent and effluent-irrigated soil, at levels suggestive of good survival in the effluent pond. Significance and Impact of the Study: This is the first study to provide quantitative information on Arcobacter spp. levels in piggery effluent and to associate A. cibarius with pigs and piggery effluent environments. |
Identificador |
Chinivasagam, H.H. and Corney, B.G. and Wright, L.L. and Diallo, I.S. and Blackall, P.J. (2007) Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland. Journal of Applied Microbiology, 103 (2). pp. 418-426. |
Publicador |
Blackwell Publishing Ltd |
Relação |
http://dx.doi.org/10.1111/j.1365-2672.2007.03275.x http://era.daf.qld.gov.au/850/ |
Palavras-Chave | #Swine #Microbiology #Soils. Soil science |
Tipo |
Article PeerReviewed |