Antibodies to guanosine: Fractionation and specificities
Data(s) |
15/10/1984
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Resumo |
Bovine serum albumin conjugates of guanosine prepared by the periodate method was used as immunogen to elicit guanosine antibodies in rabbits. The specificities of the antibodies were studied by the inhibition of their binding to [3H]Gox-red, [32P]DNA and [3H]RNA by related non-radioactive compounds. A population of antibodies is specific to Gox-red with an average association constant of around 107M−1 at 4°C. There are a population of antibodies which bind to [32P]ssDNA and [3H]RNA specifically at guanosine residues. RNA binding antibodies were separated into two populations by affinity chromatography. |
Formato |
application/pdf |
Identificador |
http://eprints.iisc.ernet.in/20391/1/1full_text.pdf Iswari, S and Jacob, TM (1984) Antibodies to guanosine: Fractionation and specificities. In: FEBS letters, 176 (1). pp. 43-48. |
Publicador |
Elsevier Science |
Relação |
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44BF14X-6V&_user=512776&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000025298&_version=1&_urlVersion=0&_userid=512776&md5=204863fa84e20af2032273a650e7bd88 http://eprints.iisc.ernet.in/20391/ |
Palavras-Chave | #Biochemistry |
Tipo |
Journal Article PeerReviewed |