3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer


Autoria(s): Huss, Wendy J.; Chambers, Karen F.; Mosaad, Eman M.O.; Russell, Pamela J.; Clements, Judith A.; Doran, Michael R.
Data(s)

07/11/2014

Resumo

Despite monolayer cultures being widely used for cancer drug development and testing, 2D cultures tend to be hypersensitive to chemotherapy and are relatively poor predictors of whether a drug will provide clinical benefit. Whilst generally more complicated, three dimensional (3D) culture systems often better recapitulate true cancer architecture and provide a more accurate drug response. As a step towards making 3D cancer cultures more accessible, we have developed a microwell platform and surface modification protocol to enable high throughput manufacture of 3D cancer aggregates. Herein we use this novel system to characterize prostate cancer cell microaggregates, including growth kinetics and drug sensitivity. Our results indicate that prostate cancer cells are viable in this system, however some non-cancerous prostate cell lines are not. This system allows us to consistently control for the presence or absence of an apoptotic core in the 3D cancer microaggregates. Similar to tumor tissues, the 3D microaggregates display poor polarity. Critically the response of 3D microaggregates to the chemotherapeutic drug, docetaxel, is more consistent with in vivo results than the equivalent 2D controls. Cumulatively, our results demonstrate that these prostate cancer microaggregates better recapitulate the morphology of prostate tumors compared to 2D and can be used for high-throughput drug testing.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/84360/

Publicador

Public Library of Science

Relação

http://eprints.qut.edu.au/84360/1/3D%20cultures%20of%20prostate%20cancer%20cells.pdf

DOI:10.1371/journal.pone.0111029

Huss, Wendy J., Chambers, Karen F., Mosaad, Eman M.O., Russell, Pamela J., Clements, Judith A., & Doran, Michael R. (2014) 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer. PLoS ONE, 9(11), e111029.

Direitos

Copyright 2014 Chambers et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Fonte

Institute of Health and Biomedical Innovation

Palavras-Chave #Prostate cancer #Cell polarity #Cell staining #Cell metabolism #Apoptosis
Tipo

Journal Article