Human glutathione S-transferase T1-1 enhances mutagenicity of 1,2-dibromoethane, dibromomethane and 1,2,3,4-diepoxybutane in Salmonella typhimurium


Autoria(s): Thier, Ricarda; Pemble, Sally E.; Kramer, Harry; Taylor, Jennifer; Guengerich, F. Peter; Ketterer, Brian
Data(s)

1996

Resumo

The rat theta class glutathione S-transferase (GST) 5-5 has been shown to affect the mutagenicity of halogenated alkanes and epoxides. In Salmonella typhimurium TA1535 expressing the rat GST5-5 the number of revertants was increased compared to the control strain by CH2Br2, ethylene dibromide (EDB) and 1,2,3,4-diepoxybutane (BDE); in contrast, mutagenicity of 1,2-epoxy-3-(4'-nitrophenoxy)propane (EPNP) was reduced. S.typhimurium TA1535 cells were transformed with an expression plasmid carrying the cDNA of the human theta ortholog GST1-1 either in sense or antisense orientation, the latter being the control. These transformed bacteria were utilized for mutagenicity assays. Mutagenicity of EDB, BDE, CH2Br2, epibromohydrin and 1,3-dichloroacetone was higher in the S.typhimurium TA1535 expressing GSTT1-1 than in the control strain. The expression of active enzyme did not affect the mutagenicity of 1,2-epoxy-3-butene or propylene oxide, GSTT1-1 expression reduced the mutagenicity of EPNP. Glutathione S-transferase 5-5 and GSTT1-1 modulate genotoxicity of several industrially important chemicals in the same way. Polymorphism of the GSTT1 locus in humans may therefore cause differences in cancer susceptibility between the two phenotypes.

Identificador

http://eprints.qut.edu.au/77492/

Publicador

Oxford University Press

Relação

DOI:10.1093/carcin/17.1.163

Thier, Ricarda, Pemble, Sally E., Kramer, Harry, Taylor, Jennifer, Guengerich, F. Peter, & Ketterer, Brian (1996) Human glutathione S-transferase T1-1 enhances mutagenicity of 1,2-dibromoethane, dibromomethane and 1,2,3,4-diepoxybutane in Salmonella typhimurium. Carcinogenesis, 17(1), pp. 163-166.

Direitos

Copyright 1996 Oxford University Press

Fonte

School of Clinical Sciences; Faculty of Health

Palavras-Chave #1 #2 dibromoethane #alkane derivative #butadiene diepoxide #complementary dna #dibromomethane #epoxide #glutathione transferase #mutagenic agent #article #cell transformation #controlled study #enzyme activity #expression vector #mutagen testing #mutagenicity #nonhuman #plasmid #priority journal #revertant #salmonella typhimurium #Animals #Epoxy Compounds #Ethylene Dibromide #Humans #Hydrocarbons #Brominated #Mutagens #Rats
Tipo

Journal Article