STAR : Predicting recombination sites from amino acid sequence
Data(s) |
08/10/2006
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Resumo |
Background Designing novel proteins with site-directed recombination has enormous prospects. By locating effective recombination sites for swapping sequence parts, the probability that hybrid sequences have the desired properties is increased dramatically. The prohibitive requirements for applying current tools led us to investigate machine learning to assist in finding useful recombination sites from amino acid sequence alone. Results We present STAR, Site Targeted Amino acid Recombination predictor, which produces a score indicating the structural disruption caused by recombination, for each position in an amino acid sequence. Example predictions contrasted with those of alternative tools, illustrate STAR'S utility to assist in determining useful recombination sites. Overall, the correlation coefficient between the output of the experimentally validated protein design algorithm SCHEMA and the prediction of STAR is very high (0.89). Conclusion STAR allows the user to explore useful recombination sites in amino acid sequences with unknown structure and unknown evolutionary origin. The predictor service is available from http://pprowler.itee.uq.edu.au/star. |
Identificador | |
Publicador |
BioMed Central Ltd. |
Relação |
DOI:10.1186/1471-2105-7-437 Bauer, Denis C., Boden, Mikael, Thier, Ricarda, & Gillam, Elizabeth M. (2006) STAR : Predicting recombination sites from amino acid sequence. BMC Bioinformatics, 7(437). |
Direitos |
Copyright 2006 Bauer et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Fonte |
School of Clinical Sciences; Faculty of Health |
Palavras-Chave | #Amino acid sequence #Correlation coefficient #Evolutionary origin #Hybrid sequences #Novel proteins #Protein design #Amino acids #Proteins #Tools #Stars #algorithm #article #artificial intelligence #controlled study #DNA recombination #Internet #prediction #probability #scoring system |
Tipo |
Journal Article |