Structure and function of DsbA, a key bacterial oxidative folding catalyst


Autoria(s): Shouldice, Stephen R.; Heras, Begoña; Walden, Patricia M.; Totsika, Makrina; Schembri, Mark A.; Martin, Jennifer L.
Data(s)

01/05/2011

Resumo

Since its discovery in 1991, the bacterial periplasmic oxidative folding catalyst DsbA has been the focus of intense research. Early studies addressed why it is so oxidizing and how it is maintained in its less stable oxidized state. The crystal structure of Escherichia coli DsbA (EcDsbA) revealed that the oxidizing periplasmic enzyme is a distant evolutionary cousin of the reducing cytoplasmic enzyme thioredoxin. Recent significant developments have deepened our understanding of DsbA function, mechanism, and interactions: the structure of the partner membrane protein EcDsbB, including its complex with EcDsbA, proved a landmark in the field. Studies of DsbA machineries from bacteria other than E. coli K-12 have highlighted dramatic differences from the model organism, including a striking divergence in redox parameters and surface features. Several DsbA structures have provided the first clues to its interaction with substrates, and finally, evidence for a central role of DsbA in bacterial virulence has been demonstrated in a range of organisms. Here, we review current knowledge on DsbA, a bacterial periplasmic protein that introduces disulfide bonds into diverse substrate proteins and which may one day be the target of a new class of anti-virulence drugs to treat bacterial infection. Antioxid. Redox Signal. 14, 1729–1760.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/77400/

Publicador

Mary Ann Liebert, Inc. Publishers

Relação

http://eprints.qut.edu.au/77400/1/Shouldice_2011_DsbA_Review_ARS.pdf

DOI:10.1089/ars.2010.3344

Shouldice, Stephen R., Heras, Begoña, Walden, Patricia M., Totsika, Makrina, Schembri, Mark A., & Martin, Jennifer L. (2011) Structure and function of DsbA, a key bacterial oxidative folding catalyst. Antioxidants & Redox Signaling, 14(9), pp. 1729-1760.

Direitos

Copyright 2011 Mary Ann Liebert, Inc.

This is a copy of an article published in Antioxidants & Redox Signaling © 2011 Mary Ann Liebert, Inc.; Antioxidants & Redox Signaling is available online at: http://online.liebertpub.com.

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Tipo

Review