Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests


Autoria(s): Baker, Joanne; Ho, Mei-Fong; Pelecanos, Anita M.; Gatton, Michelle L.; Chen, Nanhua; Abdullah, Salim; Albertini, Audrey; Ariey, Frederic; Barnwell, John; Bell, David; Cunningham, Jane; Djalle, Djibrine; Echeverry, Diego F.; Gamboa, Dionicia; Hii, Jeffery; Kyaw, Myat Phone; Luchavez, Jennifer; Membi, Christopher; Menard, Didier; Murillo, Claribel; Nhem, Sina; Ogutu, Bernhards; Onyor, Pamela; Oyibo, Wellington; Wang, Shan Qing; McCarthy, James; Cheng, Qin
Data(s)

17/05/2010

Resumo

Background Accurate diagnosis is essential for prompt and appropriate treatment of malaria. While rapid diagnostic tests (RDTs) offer great potential to improve malaria diagnosis, the sensitivity of RDTs has been reported to be highly variable. One possible factor contributing to variable test performance is the diversity of parasite antigens. This is of particular concern for Plasmodium falciparum histidine-rich protein 2 (PfHRP2)-detecting RDTs since PfHRP2 has been reported to be highly variable in isolates of the Asia-Pacific region. Methods The pfhrp2 exon 2 fragment from 458 isolates of P. falciparum collected from 38 countries was amplified and sequenced. For a subset of 80 isolates, the exon 2 fragment of histidine-rich protein 3 (pfhrp3) was also amplified and sequenced. DNA sequence and statistical analysis of the variation observed in these genes was conducted. The potential impact of the pfhrp2 variation on RDT detection rates was examined by analysing the relationship between sequence characteristics of this gene and the results of the WHO product testing of malaria RDTs: Round 1 (2008), for 34 PfHRP2-detecting RDTs. Results Sequence analysis revealed extensive variations in the number and arrangement of various repeats encoded by the genes in parasite populations world-wide. However, no statistically robust correlation between gene structure and RDT detection rate for P. falciparum parasites at 200 parasites per microlitre was identified. Conclusions The results suggest that despite extreme sequence variation, diversity of PfHRP2 does not appear to be a major cause of RDT sensitivity variation.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/75117/

Publicador

BioMed Central Ltd

Relação

http://eprints.qut.edu.au/75117/1/75117.pdf

DOI:10.1186/1475-2875-9-129

Baker, Joanne, Ho, Mei-Fong, Pelecanos, Anita M., Gatton, Michelle L., Chen, Nanhua, Abdullah, Salim, Albertini, Audrey, Ariey, Frederic, Barnwell, John, Bell, David, Cunningham, Jane, Djalle, Djibrine, Echeverry, Diego F., Gamboa, Dionicia, Hii, Jeffery, Kyaw, Myat Phone, Luchavez, Jennifer, Membi, Christopher, Menard, Didier, Murillo, Claribel, Nhem, Sina, Ogutu, Bernhards, Onyor, Pamela, Oyibo, Wellington, Wang, Shan Qing, McCarthy, James, & Cheng, Qin (2010) Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests. Malaria Journal, 9(129).

Direitos

Copyright 2010 Baker et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fonte

Faculty of Health; School of Public Health & Social Work

Palavras-Chave #110803 Medical Parasitology #111799 Public Health and Health Services not elsewhere classified #Plasmodium falciparum #histidine-rich protein #HPR2 #malaria #diagnostics #sequence analysis #rapid diagnostic tests
Tipo

Journal Article