Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix


Autoria(s): Vashi, Aditya V.; Abberton, Keren M.; Thomas, Gregory P.; Morrison, Wayne A.; O'Connor, Andrea J.; Cooper-White, Justin J.; Thompson, Erik W.
Data(s)

2006

Resumo

Adipose tissue forms when basement membrane extract (Matrigel™) and fibroblast growth factor-2 (FGF-2) are added to our mouse tissue engineering chamber model. A mouse tumor extract, Matrigel is unsuitable for human clinical application, and finding an alternative to Matrigel is essential. In this study we generated adipose tissue in the chamber model without using Matrigel by controlled release of FGF-2 in a type I collagen matrix. FGF-2 was impregnated into biodegradable gelatin microspheres for its slow release. The chambers were filled with these microspheres suspended in 60 μL collagen gel. Injection of collagen containing free FGF-2 or collagen containing gelatin microspheres with buffer alone served as controls. When chambers were harvested 6 weeks after implantation, the volume and weight of the tissue obtained were higher in the group that received collagen and FGF-2 impregnated microspheres than in controls. Histologic analysis of tissue constructs showed the formation of de novo adipose tissue accompanied by angiogenesis. In contrast, control groups did not show extensive adipose tissue formation. In conclusion, this study has shown that de novo formation of adipose tissue can be achieved through controlled release of FGF-2 in collagen type I in the absence of Matrigel.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/72601/

Publicador

Mary Ann Liebert, Inc. Publishers

Relação

http://eprints.qut.edu.au/72601/1/72601%28pub%29.pdf

DOI:10.1089/ten.2006.12.3035

Vashi, Aditya V., Abberton, Keren M., Thomas, Gregory P., Morrison, Wayne A., O'Connor, Andrea J., Cooper-White, Justin J., & Thompson, Erik W. (2006) Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix. Tissue Engineering, 12(11), pp. 3035-3043.

Direitos

Copyright 2006 Mary Ann Liebert, Inc. Publishers

Fonte

Faculty of Health

Tipo

Journal Article