Calcium influx inhibits MT1-MMP processing and blocks MMP-2 activation
Data(s) |
04/08/1997
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Resumo |
We have previously reported that concanavalin A (ConA)-induced MMP-2 activation involves both transcriptional and non-transcriptional mechanisms. Here we examined the effects of calcium influx on MT1-MMP expression and MMP-2 activation in MDA-MB-231 cells. The calcium ionophore ionomycin caused a dose-dependent inhibition of ConA-induced MMP-2 activation, but had no effect on MT1-MMP mRNA levels. However, Western analysis revealed an accumulation of pro-MT1-MMP (63 kDa), indicating that ionomycin blocked the conversion of pro-MT1-MMP protein to the active 60 kDa form. This suggests that increased calcium levels inhibit the processing of MT1-MMP. This finding may help to elucidate the mechanism(s) which regulates MT1-MMP activation. |
Identificador | |
Publicador |
Elsevier BV |
Relação |
DOI:10.1016/S0014-5793(97)00849-1 Yu, Ming, Sato, Hiroshi, Seiki, Motoharu, Spiegel, Sarah, & Thompson, Erik W. (1997) Calcium influx inhibits MT1-MMP processing and blocks MMP-2 activation. FEBS Letters, 412(3), pp. 568-572. |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #Activation #Calcium #Concanavalin A #Gelatinase A #Human breast cancer #MT-MMP |
Tipo |
Journal Article |