Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation
Data(s) |
2000
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Resumo |
We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them. |
Formato |
application/pdf |
Identificador | |
Publicador |
Optical Society of America |
Relação |
http://eprints.qut.edu.au/72142/1/72142%28pub%29.pdf http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww%2Eopticsinfobase%2Eorg%2FDirectPDFAccess%2F5195C8A5%2D06FB%2D7EBF%2D9FA54606458FD8A6%5F62709%2Fao%2D39%2D34%2D6312%2Epdf%3Fda%3D1%26id%3D62709%26seq%3D0%26mobile%3Dno&org=Queensland%2 Xu, Ming G., Williams, Elizabeth D., Thompson, Erik W., & Gu, Min (2000) Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation. Applied Optics, 39(34), pp. 6312-6317. |
Direitos |
Copyright 2000 Optical Society of America |
Fonte |
Faculty of Health |
Tipo |
Journal Article |