Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation


Autoria(s): Xu, Ming G.; Williams, Elizabeth D.; Thompson, Erik W.; Gu, Min
Data(s)

2000

Resumo

We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/72142/

Publicador

Optical Society of America

Relação

http://eprints.qut.edu.au/72142/1/72142%28pub%29.pdf

http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww%2Eopticsinfobase%2Eorg%2FDirectPDFAccess%2F5195C8A5%2D06FB%2D7EBF%2D9FA54606458FD8A6%5F62709%2Fao%2D39%2D34%2D6312%2Epdf%3Fda%3D1%26id%3D62709%26seq%3D0%26mobile%3Dno&org=Queensland%2

Xu, Ming G., Williams, Elizabeth D., Thompson, Erik W., & Gu, Min (2000) Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation. Applied Optics, 39(34), pp. 6312-6317.

Direitos

Copyright 2000 Optical Society of America

Fonte

Faculty of Health

Tipo

Journal Article