Elevated cyclic AMP suppresses ConA-induced MT1-MMP expression in MDA- MB-231 human breast cancer cells


Autoria(s): Yu, M.; Sato, H.; Seiki, M.; Spiegel, S.; Thompson, E.W.
Data(s)

1998

Resumo

We have previously reported that induction of MMP-2 activation by Concanavalin A (ConA) in MDA-MB-231 human breast cancer cells involves both transcriptional and post-transcriptional mechanisms, and that the continuous presence of ConA is required for MMP-2 activation (Yu et al. Cancer Res, 55, 3272-7, 1995). In an effort to identify signal transduction pathways which may either contribute to or modulate this mechanism, we found that three different cAMP-inducing agents, cholera toxin (CT), forskolin (FSK), and 3- isobutyl-1-methylxanthine (IBMX) partially inhibited ConA-induced MT1-MMP expression and MMP-2 activation in MDA-MB-231 cells. Combinations of CT or FSK with IBMX exhibited additive effects on reduction of MT1-MMP mRNA expression and MMP-2 activation. Agents which increase cAMP levels appeared to target transcriptional aspects of ConA induction, reducing MT1-MMP mRNA and protein in parallel with the reduced MMP-2 activation. In the absence of ConA, down-regulation of constitutive production of MT1-MMP mRNA and protein was observed, indicating that cAMP acts independently of ConA. These observations may help to elucidate factors regulating MT1-MMP expression, which may be pivotal to the elaboration of invasive machinery on the cell surface.

Identificador

http://eprints.qut.edu.au/71920/

Publicador

Springer Verlag

Relação

DOI:10.1023/A:1006580406314

Yu, M., Sato, H., Seiki, M., Spiegel, S., & Thompson, E.W. (1998) Elevated cyclic AMP suppresses ConA-induced MT1-MMP expression in MDA- MB-231 human breast cancer cells. Clinical and Experimental Metastasis, 16(2), pp. 185-191.

Fonte

Faculty of Health

Palavras-Chave #Activation #cAMP #Concanavalin A #Gelatinasa A #Human breast cancer #MT-MMP
Tipo

Journal Article