Physiological basis of salt stress tolerance in rice expressing the antiapoptotic gene SfIAP


Autoria(s): Hoang, Thi My Linh; Williams, Brett; Khanna, Harjeet; Dale, James; Mundree, Sagadevan G.
Data(s)

01/02/2014

Resumo

Programmed cell death-associated genes, especially antiapoptosis-related genes have been reported to confer tolerance to a wide range of biotic and abiotic stresses in dicotyledonous plants such as tobacco (Nicotiana tabacum L.) and tomato (Solanum lycopersicum L.). This is the first time the antiapoptotic gene SfIAP was transformed into a monocotyledonous representative: rice (Oryza sativa L.). Transgenic rice strains expressing SfIAP were generated by the Agrobacterium-mediated transformation method and rice embryogenic calli, and assessed for their ability to confer tolerance to salt stress at both the seedling and reproductive stages using a combination of molecular, agronomical, physiological and biochemical techniques. The results show that plants expressing SfIAP have higher salt tolerance levels in comparison to the wild-type and vector controls. By preventing cell death at the onset of salt stress and maintaining the cell membrane’s integrity, SfIAP transgenic rice plants can retain plant water status, ion homeostasis, photosynthetic efficiency and growth to combat salinity successfully.

Identificador

http://eprints.qut.edu.au/71009/

Publicador

C S I R O Publishing

Relação

DOI:10.1071/FP13308

Hoang, Thi My Linh, Williams, Brett, Khanna, Harjeet, Dale, James, & Mundree, Sagadevan G. (2014) Physiological basis of salt stress tolerance in rice expressing the antiapoptotic gene SfIAP. Functional Plant Biology, 41(11), pp. 1168-1177.

Fonte

Centre for Tropical Crops and Biocommodities; Faculty of Science and Technology; Institute for Future Environments; Science & Engineering Faculty

Palavras-Chave #060705 Plant Physiology #programmed cell death #terminal doxynucleotidyl transferase d-UTP nick end labelling #TUNEL #Apoptosis #Salinity
Tipo

Journal Article