Metabolomic analysis of siderophore cheater mutants reveals metabolic costs of expression in uropathogenic escherichia coli


Autoria(s): Lv, Haitao; Hung, Chia S.; Henderson, Jeffrey P.
Data(s)

2014

Resumo

Bacterial siderophores are a group of chemically diverse, virulence-associated secondary metabolites whose expression exerts metabolic costs. A combined bacterial genetic and metabolomic approach revealed differential metabolomic impacts associated with biosynthesis of different siderophore structural families. Despite myriad genetic differences, the metabolome of a cheater mutant lacking a single set of siderophore biosynthetic genes more closely approximate that of a nonpathogenic K12 strain than its isogenic, uropathogen parent strain. Siderophore types associated with greater metabolomic perturbations are less common among human isolates, suggesting that metabolic costs influence success in a human population. Although different siderophores share a common iron acquisition function, our analysis shows how a metabolomic approach can distinguish their relative metabolic impacts in E.coli.

Identificador

http://eprints.qut.edu.au/66825/

Publicador

American Chemical Society

Relação

DOI:10.1021/pr4009749

Lv, Haitao, Hung, Chia S., & Henderson, Jeffrey P. (2014) Metabolomic analysis of siderophore cheater mutants reveals metabolic costs of expression in uropathogenic escherichia coli. Journal of Proteome Research, 13(3), pp. 1397-1404.

Direitos

Copyright 2014 American Chemical Society

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Tipo

Journal Article