Stored blood products augment inflammation in a two-event model of Transfusion Related Acute Lung Injury (TRALI)


Autoria(s): Dean, Melinda M.; Bierman, Wesley; Knauth, Christine; Flower, Robert L.; Tung, John-Paul
Contribuinte(s)

Mills, Tony

Data(s)

01/10/2013

Resumo

Aim/Background TRALI is hypothesised to develop via a two-event mechanism involving both the patieint's underlying morbidity and blood product factors. The storage of cellular products has been implicated in cases of non-antibody mediated TRALI, however the pathophysiological mechanisms are undefined. We investigated blood product storage-related modulation of inflmmatory cells and medicators involved in TRALI. Methods In an in vitro mode, fresh human whole blood was mixed with culture media (control) or LPS as a 1st event and "transfused" with 10% (v/v) pooled supernatant (SN) from Day 1 (d1, n=75) or Day 42 (D42, n=113) packed red blood cells (PRBCs) as a 2nd event. Following 6hrs, culture SN was used to assess the overall inflammatory response (cytometric bead array) and a duplicate assay containing protein transport inhibitor was used to assess neutrophil- and monocyte-specific inflmamatory responses using multi-colour flow cytometry. Panels: IL-6, IL-8, IL-10, IL-12, IL-1, TNF, MCP-1, IP-10, MIP-1. One-way ANOVA 95% CI. Results In the absence of LPS, exposure to D1 or D42 PRBC-SN reduced monocyte expression of IL-6, IL-8 and Il-10. D42 PRBC-SN also reduced monocyte IP-10, and the overall IL-8 production was increased. In the presence of LPS, D1-PRBC SN only modified overall IP-10 levels which were reduced. However, cf LPS alone, the combination of LPS and D42 PRBC-SN resulted in increased neutrophil and monocyte productionof IL-1 and IL-8 as well as reduced monocyte TNF production. Additionally, LPS and D42 PRBC-SN resulted in overall inflmmatory changes: elevated IL-8, <IP-1, IL-10 and MIP-1, and reduced TNF and IP-10. Conclusion In the absence of LPS, exposure to both fresh and stored PRBC-SN predominantly resulted in an anti-imflammatory response. However, in the presence of LPS, stored but not fresh PRBC-SN augmented a pro-inflammatory profile. These data support the two-event mechanism of TRALI pathogenesis, and highlights blood product storage duration as a factor contributing to the development of inflmamatory responses in non-antibody mediated TRALI.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/66718/

Relação

http://eprints.qut.edu.au/66718/1/HAA_P006_StoredBloodTRALI.pdf

Dean, Melinda M., Bierman, Wesley, Knauth, Christine, Flower, Robert L., & Tung, John-Paul (2013) Stored blood products augment inflammation in a two-event model of Transfusion Related Acute Lung Injury (TRALI). In Mills, Tony (Ed.) HAA 2013, 20-23 October 2013, Gold Coast, QLD, Australia . (Unpublished)

Direitos

Copyright 2013 Please consult the authors

Fonte

School of Biomedical Sciences; School of Chemistry, Physics & Mechanical Engineering; Faculty of Health

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #Transfusion-related lung injury #Inflammation #Stored blood
Tipo

Conference Item