Rice ragged stunt oryzavirus genome segment S4 could encode an RNA dependent RNA polymerase and a second protein of unknown function


Autoria(s): Upadhyaya, N. M.; Ramm, K.; Gellatly, J. A.; Li, Z.; Kositratana, W.; Waterhouse, Peter M.
Data(s)

1998

Resumo

The complete nucleotide sequence of genome segment S4 of rice ragged stunt oryzavirus (RRSV, Thai-isolate) was determined. The 3823 bp sequence contains two large open reading frames (ORFs). ORF1, spanning nucleotides 12 to 3776, is capable of encoding a protein of M(r) 141,380 (P4a). The P4a amino acid sequence predicted from the nucleotide sequence contains sequence motifs conserved in RNA-dependent RNA polymerases (RDRPs). When compared for evolutionary relationships with RDRPs of other reoviruses using the amino acid sequences around the conserved GDD motif, P4a was shown to be more related to Nilaparvata lugens reovirus and reovirus serotype 3 than to rice dwarf phytoreovirus, bovine rotavirus or bluetongue virus. The ORF2, spanning nucleotides 491 to 1468, is out of frame with ORF1 and is capable of encoding a protein of 36, 920 (P4b). Coupled in vitro transcription-translation from cloned ORF2 in wheat germ extract confirmed the existence of ORF2 but in vivo production and possible function of P4b is yet to be determined.

Identificador

http://eprints.qut.edu.au/66004/

Publicador

Springer Wien

Relação

DOI:10.1007/s007050050419

Upadhyaya, N. M., Ramm, K., Gellatly, J. A., Li, Z., Kositratana, W., & Waterhouse, Peter M. (1998) Rice ragged stunt oryzavirus genome segment S4 could encode an RNA dependent RNA polymerase and a second protein of unknown function. Archives of Virology, 143(9), pp. 1815-1822.

Direitos

Copyright 1998 Springer Wien

Fonte

School of Earth, Environmental & Biological Sciences; Science & Engineering Faculty

Palavras-Chave #060400 GENETICS #060700 PLANT BIOLOGY #bluetongue orbivirus #bovine rotavirus #genetic analysis #Nilaparvata lugens #reovirus #rice dwarf phytoreovirus #rice ragged stunt oryzavirus #RNA polymerase #Amino Acid Sequence #Base Sequence #Genome #Viral #Molecular Sequence Data #Open Reading Frames #Oryza sativa #Reoviridae #RNA Replicase #Viral Proteins #Bluetongue virus #Bovinae #Nilaparvata lugens reovirus #Orthoreovirus #Reovirus sp. #Rice dwarf virus #Rice ragged stunt virus #Rotavirus #Triticum aestivum
Tipo

Journal Article