Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development


Autoria(s): Duan, Guowen; Saint, Robert B.; Helliwell, Chris A.; Behm, Carolyn A.; Waterhouse, Peter M.; Gordon, Karl H. J.
Data(s)

01/12/2010

Resumo

Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT-PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development. © 2010 Springer Science+Business Media B.V.

Identificador

http://eprints.qut.edu.au/65915/

Publicador

Springer NVerlag

Relação

DOI:10.1007/s11248-010-9372-y

Duan, Guowen, Saint, Robert B., Helliwell, Chris A., Behm, Carolyn A., Waterhouse, Peter M., & Gordon, Karl H. J. (2010) Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development. Transgenic Research, 19(6), pp. 1121-1128.

Direitos

Copyright 2010 Springer Verlag

Fonte

School of Earth, Environmental & Biological Sciences; Science & Engineering Faculty

Palavras-Chave #060400 GENETICS #060700 PLANT BIOLOGY #MicroRNAs (miRNAs) #RNA interference (RNAi) #RNA-directed RNA polymerase (RdRp) #Caenorhabditis elegans protein #EGO 1 protein #C elegans #EGO-1 protein #C elegans #helminth DNA #recombinant protein #RNA directed RNA polymerase #animal #article #Caenorhabditis elegans #Drosophila melanogaster #enzymology #female #gene #gene expression #genetics #growth #development and aging #male #metabolism #morphogenesis #nucleotide sequence #phylogeny #physiology #RNA interference #transgenic animal #Animals #Animals #Genetically Modified #Base Sequence #Caenorhabditis elegans Proteins #DNA #Helminth #Genes #Helminth #Recombinant Proteins #RNA Replicase #Hexapoda #Vertebrata
Tipo

Journal Article