Matrix metalloproteinases 2 and 9 (gelatinases A and B) expression in malignant mesothelioma and benign pleura
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2003
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Resumo |
Matrix metalloproteinases (MMPs), in particular the gelatinases (MMP-2 and -9), play a significant role in tumour invasion and angiogenesis. The expression and activities of MMPs have not been characterised in malignant mesothelioma (MM) tumour samples. In a prospective study, gelatinase activity was evaluated in homogenised supernatants of snap frozen MM (n = 35), inflamed pleura (IP, n = 12) and uninflammed pleura (UP, n = 14) tissue specimens by semiquantitative gelatin zymography. Matrix metalloproteinases were correlated with clinicopathological factors and with survival using Kaplan-Meier and Cox proportional hazard models. In MM, pro- and active MMP-2 levels were significantly greater than for MMP-9 (P = 0.006, P<0.001). Active MMP-2 was significantly greater in MM than in UP (P=0.04). MMP-2 activity was equivalent between IP and MM, but both pro- and active MMP-9 activities were greater in IP (P=0.02, P=0.009). While there were trends towards poor survival with increasing total and pro-MMP-2 activity (P=0.08) in univariate analysis, they were both independent poor prognostic factors in multivariate analysis in conjunction with weight loss (pro-MMP-2 P = 0.03, total MMP-2 P = 0.04). Total and pro-MMP-2 also contributed to the Cancer and Leukemia Group B prognostic groups. MMP-9 activities were not prognostic. Matrix metalloproteinases, and in particular MMP-2, the most abundant gelatinase, may play an important role in MM tumour growth and metastasis. Agents that reduce MMP synthesis and/or activity may have a role to play in the management of MM. © 2003 Cancer Research UK. |
Identificador | |
Publicador |
Nature Publishing Group |
Relação |
DOI:10.1038/sj.bjc.6600920 Edwards, J. G., McLaren, J., Jones, J. L., Waller, D. A., & O'Byrne, Kenneth J. (2003) Matrix metalloproteinases 2 and 9 (gelatinases A and B) expression in malignant mesothelioma and benign pleura. British Journal of Cancer : BJC, 88(10), pp. 1553-1559. |
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Copyright 2003 Nature Publishing Group |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #Malignant mesothelioma #Matrix metalloproteinases #Prognosis #gelatinase A #gelatinase B #angiogenesis #article #benign tumor #cancer invasion #cancer survival #controlled study #human #human tissue #Kaplan Meier method #metastasis #multivariate analysis #pleura tumor #pleurisy #priority journal #protein expression #tumor growth #zymography #Adult #Aged #Disease Progression #Gene Expression Regulation #Neoplastic #Humans #Immunohistochemistry #Matrix Metalloproteinase 2 #Matrix Metalloproteinase 9 #Mesothelioma #Middle Aged #Neoplasm Metastasis #Prospective Studies #Survival |
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Journal Article |