Identification and simultaneous analysis of harmane, harmol, isovitexin and vitexin in Passiflora incarnata extracts with a novel HLPC method
Data(s) |
2001
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Resumo |
A high performance liquid chromatographic method for the simultaneous analysis of two flavonoids (iso-vitexin and vitexin), and three indole alkaloids (harmane, harmine, and harmol) was developed. This method was then utilised to quantitate levels of these five constituents in methanolic extracts of Australian Passiflora incarnata. HPLC analysis was performed using a Waters™ Novapak C18 (150 × 4 mm, 4 μm) column, with a gradient solvent system of methanol-water-acetic acid. Detection was achieved by PDA UV (254 nm) and fluorescence (excitation 254 nm, emission 414 nm), utilising the external standard method to obtain quantification. |
Identificador | |
Publicador |
Taylor & Francis Inc. |
Relação |
DOI:10.1081/JLC-100105957 Grice, I. D., Ferreira, L. A., & Griffiths, Lyn R. (2001) Identification and simultaneous analysis of harmane, harmol, isovitexin and vitexin in Passiflora incarnata extracts with a novel HLPC method. Journal of Liquid Chromatography & Related Technologies, 24(16), pp. 2513-2523. |
Direitos |
Copyright 2001 Taylor & Francis Inc. |
Fonte |
Institute of Health and Biomedical Innovation |
Tipo |
Journal Article |