Paclitaxel resistance and multicellular spheroid formation are induced by kallikrein-related peptidase 4 in serous ovarian cancer cells in an ascites mimicking microenvironment


Autoria(s): Dong, Ying; Stephens, Carson; Walpole, Carina; Swedberg, Joakim; Boyle, Glen M.; Parsons, Peter G.; McGuckin, Michael A.; Harris, Jonathan; Clements, Judith
Data(s)

25/02/2013

Resumo

High tumor kallikrein-related-peptidase 4 (KLK4) levels are associated with a poor outcome for women with serous epithelial ovarian cancer (EOC), for which peritoneal dissemination and chemoresistance are key events. To determine the role of KLK4 in these events, we examined KLK4-transfected SKOV-3 and endogenous KLK4 expressing OVCA432 cells in 3-dimensional (3D) suspension culture to mimic the ascites microenvironment. KLK4-SKOV-3 cells formed multicellular aggregates (MCAs) as seen in ascites, as did SKOV-3 cells treated with active KLK4. MCA formation was reduced by treatment with a KLK4 blocking antibody or the selective active site KLK4 sunflower trypsin inhibitor (SFTI-FCQR). KLK4-MCAs formed larger cancer cell foci in mesothelial cell monolayers than those formed by vector and native SKOV-3 cells, suggesting KLK4-MCAs are highly invasive in the peritoneal microenvironment. A high level of KLK4 is expressed by ascitic EOC cells compared to matched primary tumor cells, further supporting its role in the ascitic microenvironment. Interestingly, KLK4 transfected SKOV-3 cells expressed high levels of the KLK4 substrate, urokinase plasminogen activator (uPA), particularly in 3D-suspension, and high levels of both KLK4 and uPA were observed in patient cells taken from ascites. Importantly, the KLK4-MCAs were paclitaxel resistant which was reversed by SFTI-FCQR and to a lesser degree by the general serine protease inhibitor, Aprotinin, suggesting that in addition to uPA, other as yet unidentified substrates of KLK4 must be involved. Nonetheless, these data suggest that KLK4 inhibition, in conjunction with paclitaxel, may improve the outcome for women with serous epithelial ovarian cancer and high KLK4 levels in their tumors.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/57799/

Publicador

Public Library of Science

Relação

http://eprints.qut.edu.au/57799/1/journal.pone.2013Dong.pdf

http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057056

DOI:10.1371/journal.pone.0057056

Dong, Ying, Stephens, Carson, Walpole, Carina, Swedberg, Joakim, Boyle, Glen M., Parsons, Peter G., McGuckin, Michael A., Harris, Jonathan, & Clements, Judith (2013) Paclitaxel resistance and multicellular spheroid formation are induced by kallikrein-related peptidase 4 in serous ovarian cancer cells in an ascites mimicking microenvironment. PLOS One, 8(2), e57056.

http://purl.org/au-research/grants/NHMRC/242220, 390123, and 550523

Direitos

Copyright: 2013 Dong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Palavras-Chave #060000 BIOLOGICAL SCIENCES #110000 MEDICAL AND HEALTH SCIENCES
Tipo

Journal Article