Polarized fluid movement and not cell death, creates luminal spaces in adult prostate epithelium


Autoria(s): Pearson, J F; Hughes, S; Chambers, Karen F.; Lang, S H
Data(s)

19/12/2008

Resumo

There are two predominant theories for lumen formation in tissue morphogenesis: cavitation driven by cell death, and membrane separation driven by epithelial polarity. To define the mechanism of lumen formation in prostate acini, we examined both theories in several cell lines grown in three-dimensional (3D) Matrigel culture. Lumen formation occurred early in culture and preceded the expression of cell death markers for apoptosis (active caspase 3) and autophagy (LC-3). Active caspase 3 was expressed by very few cells and inhibition of apoptosis did not suppress lumen formation. Despite LC-3 expression in all cells within a spheroid, this was not associated with cell death. However, expression of a prostate-secretory protein coincided with lumen formation and subsequent disruption of polarized fluid movement led to significant inhibition of lumen formation. This work indicates that lumen formation is driven by the polarized movement of fluids and proteins in 3D prostate epithelial models and not by cavitation.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/55863/

Publicador

Nature Publishing Group

Relação

http://eprints.qut.edu.au/55863/3/pearson_2010.pdf

DOI:10.1038/cdd.2008.181

Pearson, J F, Hughes, S, Chambers, Karen F., & Lang, S H (2008) Polarized fluid movement and not cell death, creates luminal spaces in adult prostate epithelium. Cell Death and Differentiation, 16(3), pp. 475-482.

Direitos

Copyright 2008 Nature Publishing Group

Fonte

Institute of Health and Biomedical Innovation

Palavras-Chave #060103 Cell Development Proliferation and Death #060106 Cellular Interactions (incl. Adhesion Matrix Cell Wall) #Prostate #Polarity #Development #Lumen formation #Apoptosis
Tipo

Journal Article