Biochemical profiling of proteins and metabolites in wound exudate from chronic wound environments


Autoria(s): Broszczak, Daniel; Stupar, Dario; Compay, Arnulf Lloyd Lucero; Sharma, Masti Venugopal Srihari; Parker, Tony J.; Shooter, Gary K.; Upton, Zee; Fernandez, Melissa Laura
Data(s)

01/06/2012

Resumo

The lack of fundamental knowledge on the biological processes associated with wound healing represents a significant challenge. Understanding the biochemical changes that occur within a chronic wound could provide insights into the wound environment and enable more effective wound management. We report on the stability of wound fluid samples under various conditions and describe a high-throughput approach to investigate the altered biochemical state within wound samples collected from various types of chronic, ulcerated wounds. Furthermore, we discuss the viability of this approach in the early stages of wound sample protein and metabolite profiling and subsequent biomarker discovery. This approach will facilitate the detection of factors that may correlate with wound severity and/or could be used to monitor the response to a particular treatment.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/54830/

Publicador

Cambridge Publishing

Relação

http://eprints.qut.edu.au/54830/1/Broszczak_2012_WPR_Accepted.pdf

http://www.awma.com.au/journal/2002.php

Broszczak, Daniel, Stupar, Dario, Compay, Arnulf Lloyd Lucero, Sharma, Masti Venugopal Srihari, Parker, Tony J., Shooter, Gary K., Upton, Zee, & Fernandez, Melissa Laura (2012) Biochemical profiling of proteins and metabolites in wound exudate from chronic wound environments. Wound Practice and Research, 20(2), pp. 62-72.

Direitos

Copyright 2012 Cambridge Publishing

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Palavras-Chave #110101 Medical Biochemistry - Amino Acids and Metabolites #110106 Medical Biochemistry - Proteins and Peptides (incl. Medical Proteomics) #110302 Clinical Chemistry (diagnostics) #Wound #Biomarker #Diagnostic #Proteomic #Metabolomic #Chronic Wound #Skin
Tipo

Journal Article