Preparation, characterization and in vitro angiogenic capacity of cobalt substituted β-tricalcium phosphate ceramics
Data(s) |
01/08/2012
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Resumo |
Divalent cobalt ions (Co2+) have been shown to possess the capacity to induce angiogenesis by activating hypoxia inducible factor-1α (HIF-1α) and subsequently inducing the production of vascular endothelial growth factor (VEGF). However, there are few reports about Co-containing biomaterials for inducing in vitro angiogenesis. The aim of the present work was to prepare Co-containing β-tricalcium phosphate (Co-TCP) ceramics with different contents of calcium substituted by cobalt (0, 2, 5 mol%) and to investigate the effect of Co substitution on their physicochemical and biological properties. Co-TCP powders were synthesized by a chemistry precipitation method and Co-TCP ceramics were prepared by sintering the powder compacts. The effect of Co substitution on phase transition and the sintering property of the β-TCP ceramics was investigated. The proliferation and VEGF expression of human bone marrow mesenchymal stem cells (HBMSCs) cultured with both powder extracts and ceramic discs of Co-TCP was further evaluated. The in vitro angiogenesis was evaluated by the tube-like structure formation of human umbilical vein endothelial cells (HUVECs) cultured on ECMatrix™ in the presence of powder extracts. The results showed that Co substitution suppressed the phase transition from β- to α-TCP. Both the powder extracts and ceramic discs of Co-TCP had generally good cytocompatibility to support HBMSC growth. Importantly, the incorporation of Co into β-TCP greatly stimulated VEGF expression of HBMSCs and Co-TCP showed a significant enhancement of network structure formation of HUVECs compared with pure TCP. Our results suggested that the incorporation of Co into bioceramics is a potential viable way to enhance angiogenic properties of biomaterials. Co-TCP bioceramics may be used for bone tissue regeneration with improved angiogenic capacity. |
Identificador | |
Publicador |
Royal Society of Chemistry |
Relação |
DOI:10.1039/c2jm34395a Zhang, Meili, Wu , Chengtie, Li , Haiyan, Yuen, Jones, Chang, Jiang, & Xiao, Yin (2012) Preparation, characterization and in vitro angiogenic capacity of cobalt substituted β-tricalcium phosphate ceramics. Journal of Materials Chemistry, 22(40), pp. 21686-21694. |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Palavras-Chave | #060000 BIOLOGICAL SCIENCES #090301 Biomaterials #In vitro angiogenic #Cobalt #HIF-1α #Co-TCP #β-TCP |
Tipo |
Journal Article |