Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism
Data(s) |
1997
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Resumo |
Significant cleavage by hammerhead ribozymes requires activation by divalent metal ions. Several models have been proposed to account for the influence of metal ions on hammerhead activity. A number of recent papers have presented data that have been interpreted as supporting a one-metal-hydroxide-ion mechanism. In addition, a solvent deuterium isotope effect has been taken as evidence against a proton transfer in the rate-limiting step of the cleavage reaction. We propose that these data are more easily explained by a two-metal-ion mechanism that does not involve a metal hydroxide, but does involve a proton transfer in the rate-limiting step. |
Formato |
application/pdf |
Identificador | |
Publicador |
National Academy of Sciences |
Relação |
http://eprints.qut.edu.au/52754/1/52754P.pdf http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9122187 Pontius, B. W., Lott, W. B., & von Hippel, P. H. (1997) Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism. Proceedings of the National Academy of Sciences of the United States of America, 94(6), pp. 2290-4. |
Direitos |
Copyright 1997 National Academy of Sciences of the USA |
Fonte |
School of Chemistry, Physics & Mechanical Engineering; Institute of Health and Biomedical Innovation; Science & Engineering Faculty |
Palavras-Chave | #Base Sequence #Binding Sites #Calcium #Catalysis #*Cations #Divalent #Cobalt #Crystallography #X-Ray #Kinetics #Lead #Magnesium #Manganese #Molecular Sequence Data #Nucleic Acid Conformation #RNA #Catalytic/*chemistry/*metabolism #Thionucleotides |
Tipo |
Journal Article |