A mass spectrometric assay for the quantification of neuropeptide PYY in plasma
Data(s) |
01/12/2012
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Resumo |
A multiple reaction monitoring mass spectrometric assay for the quantification of PYY in human plasma has been developed. A two stage sample preparation protocol was employed in which plasma containing the full length neuropeptide was first digested using trypsin, followed by solid-phase extraction to extract the digested peptide from the complex plasma matrix. The peptide extracts were analysed by LC-MS using multiple reaction monitoring to detect and quantify PYY. The method has been validated for plasma samples, yielding linear responses over the range 5–1,000 ng mL−1. The method is rapid, robust and specific for plasma PYY detection. |
Identificador | |
Publicador |
Royal Society of Chemistry |
Relação |
DOI:10.1039/C2AY05536H Xi, Li, Jin, Yaping, Josh, Peter, Parker, Edward A., Herzog, Herbert, & Colgrave, Michelle (2012) A mass spectrometric assay for the quantification of neuropeptide PYY in plasma. Analytical Methods, 4(3), pp. 714-720. |
Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
Palavras-Chave | #030000 CHEMICAL SCIENCE #spectrometric assay #PYY #plasma |
Tipo |
Journal Article |