High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni
| Data(s) |
2007
|
|---|---|
| Resumo |
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species |
| Identificador | |
| Publicador |
American Society for Microbiology |
| Relação |
DOI:10.1128/AEM.02702-06 Price, Erin P., Smith, Helen, Huygens, Flavia, & Giffard, Philip M. (2007) High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni. Applied and Environmental Microbiology, 73(10), pp. 3431-3436. |
| Fonte |
School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation |
| Palavras-Chave | #060500 MICROBIOLOGY #High Resolution Melt #Genotyping #Campylobacter #CrispR |
| Tipo |
Journal Article |
