A cell migration device that maintains a defined surface with no cellular damage during wound edge generation


Autoria(s): Doran, Michael; Mills, Richard; Parker, Anthony; Landman, Kerry; Cooper-White, Justin
Data(s)

2009

Resumo

Studying the rate of cell migration provides insight into fundamental cell biology as well as a tool to assess the functionality of synthetic surfaces and soluble environments used in tissue engineering. The traditional tools used to study cell migration include the fence and wound healing assays. In this paper we describe the development of a microchannel based device for the study of cell migration on defined surfaces. We demonstrate that this device provides a superior tool, relative to the previously mentioned assays, for assessing the propagation rate of cell wave fronts. The significant advantage provided by this technology is the ability to maintain a virgin surface prior to the commencement of the cell migration assay. Here, the device is used to assess rates of mouse fibroblasts (NIH 3T3) and human osteosarcoma (SaOS2) cell migration on surfaces functionalized with various extracellular matrix proteins as a demonstration that confining cell migration within a microchannel produces consistent and robust data. The device design enables rapid and simplistic assessment of multiple repeats on a single chip, where surfaces have not been previously exposed to cells or cellular secretions.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/42874/

Publicador

Royal Society of Chemistry

Relação

http://eprints.qut.edu.au/42874/1/42874.pdf

DOI:10.1039/b900791a

Doran, Michael, Mills, Richard, Parker, Anthony, Landman, Kerry, & Cooper-White, Justin (2009) A cell migration device that maintains a defined surface with no cellular damage during wound edge generation. Lab on a Chip, 9(16), pp. 2364-2369.

Fonte

School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation

Palavras-Chave #060106 Cellular Interactions (incl. Adhesion Matrix Cell Wall) #090301 Biomaterials #100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) #Cell Migration, Wound Assey, Cell-Surface Interactions, Micro-Channel Device, Cell Diffusion Coefficients
Tipo

Journal Article