Enhancing vascularized bone formation using tissue engineered periosteum


Autoria(s): Fan, Wei; Crawford, Ross W.; Xiao, Yin
Data(s)

01/11/2010

Resumo

Objectives: The periosteum plays an indispensable role in both bone formation and bone defect healing. The aim of this project is to produce tissue engineered periosteum for bone defect treatment. Methods: In this study we constructed an artificial in vitro periosteum by incorporating osteogenic differentiated bone marrow stromal cells (BMSCs) and cobalt chloride (CoCl2)-treated BMSCs. The engineered periostea were implanted both subcutaneously and into skull bone defects in SCID mice to investigate ectopic and orthotopic osteogenesis and vascularisation. After two weeks in subcutaneous and four weeks in bone defect areas, the implanted constructs were assessed for ectopic and orthotopic osteogenesis and vascularisation by micro-CT, histomorphometrical and immunohistochemical methods. Results: The results showed that CoCl2 pre-treated BMSCs induced higher degree of vascularisation and enhanced osteogenesis within the implants in both ectopic and orthotopic areas. Conclusion: This study provided a novel approach using BMSCs sourced from the same patient for both osteogenic and pro-angiogenic purposes in constructing tissue engineered periosteum to enhance vascularized osteogenesis.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/41137/

Relação

http://eprints.qut.edu.au/41137/1/41137.pdf

http://iadr.confex.com/iadr/2010barce/preliminaryprogram/abstract_131611.htm

Fan, Wei, Crawford, Ross W., & Xiao, Yin (2010) Enhancing vascularized bone formation using tissue engineered periosteum. In International Association for Dental Research 2010, 14-17 July 2010, Barcelona.

Direitos

Copyright 2010 [please consult the authors]

Fonte

Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation; School of Engineering Systems

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #periosteum #bone #cobalt chloride #bone marrow stromal cells #osteogenesis
Tipo

Conference Paper