A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP


Autoria(s): Pagan, Julia K.; Arnold, Jeremy; Hanchard, Kim J.; Kumar, Raman; Bruno, Tiziana; Jones, Mathew J. K.; Richard, Derek J.; Forrest, Alistair; Spurdle, Amanda B.; Verdin, Eric; Crossley, Merlin; Fanciulli, Maurizio; Chenevix-Trench, Georgia; Young, David B.; Khanna, Kum Kum
Data(s)

2007

Resumo

Corepressors play a crucial role in negative gene regulation and are defective in several diseases. BCoR is a corepressor for the BCL6 repressor protein. Here we describe and functionally characterize BCoR-L1, a homolog of BCoR. When tethered to a heterologous promoter, BCoR-L1 is capable of strong repression. Like other corepressors, BCoR-L1 associates with histone deacetylase (HDAC) activity. Specifically, BCoR-L1 coprecipitates with the Class II HDACs, HDAC4, HDAC5, and HDAC7, suggesting that they are involved in its role as a transcriptional repressor. BCoR-L1 also interacts with the CtBP corepressor through a CtBP-interacting motif in its amino terminus. Abrogation of the CtBP binding site within BCoR-L1 partially relieves BCoR-L1-mediated transcriptional repression. Furthermore, BCoR-L1 is located on the E-cadherin promoter, a known CtBP-regulated promoter, and represses the E-cadherin promoter activity in a reporter assay. The inhibition of BCoR-L1 expression by RNA-mediated interference results in derepression of E-cadherin in cells that do not normally express E-cadherin, indicating that BCoR-L1 contributes to the repression of an authentic endogenous CtBP target.

Identificador

http://eprints.qut.edu.au/40610/

Publicador

American Society for Biochemistry and Molecular Biology

Relação

DOI:10.1074/jbc.M700246200

Pagan, Julia K., Arnold, Jeremy, Hanchard, Kim J., Kumar, Raman, Bruno, Tiziana, Jones, Mathew J. K., Richard, Derek J., Forrest, Alistair, Spurdle, Amanda B., Verdin, Eric, Crossley, Merlin, Fanciulli, Maurizio, Chenevix-Trench, Georgia, Young, David B., & Khanna, Kum Kum (2007) A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP. Journal of Biological Chemistry, 282(20), pp. 15248-15257.

Direitos

Copyright 2007 American Society for Biochemistry and Molecular Biology

Fonte

Faculty of Science and Technology

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #negative gene regulation #BCL6 repressor protein #BCoR-L1
Tipo

Journal Article