Differentiation of Rice Tungro Bacilliform Virus Strains by Restriction Analysis and DNA Hybridization


Autoria(s): Azzam, O.; Cabauatan, P.Q.; Arboleda, M.
Data(s)

1998

Resumo

Rice tungro bacilliform virus (RTBV) is one of the two viruses that cause tungro disease. Four RTBV strains maintained in the greenhouse for 4 years, G1, G2, Ic, and L, were differentiated by restriction fragment length polymorphism (RFLP) analysis of the native viral DNA. Although strains G1 and Ic had identical restriction patterns when cleaved with Pst1, BamHI, EcoRI, and EcoRV, they can be differentiated from strains G2 and L by EcoRI and EcoRV digestion. These same endonucleases also differentiate strain G2 from strain L. When total DNA extracts from infected plants were used instead of viral DNA, and digested with EcoRV, identical restriction patterns for each strain (G2 and L) were obtained from roots, leaves, and leaf sheaths of infected plants. The restriction patterns were consistent from plant to plant, in different varieties, and at different times after inoculation. This technique can be used to differentiate RTBV strains and determine the variability of a large number of field samples.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/39800/

Publicador

Elsevier

Relação

http://eprints.qut.edu.au/39800/1/39800.pdf

DOI:10.1016/S0166-0934(98)00132-3

Azzam, O., Cabauatan, P.Q., & Arboleda, M. (1998) Differentiation of Rice Tungro Bacilliform Virus Strains by Restriction Analysis and DNA Hybridization. Journal of Virological Methods, 76(1-2), pp. 121-126.

Direitos

Copyright © 1998 Elsevier Science B.V. All rights reserved.

Fonte

Faculty of Science and Technology

Palavras-Chave #070600 HORTICULTURAL PRODUCTION #Rice tungro #Bacilliform virus strains #DNA hybridization #Restriction analysis #Jefferson, Osmat, Ossmat, Azzam
Tipo

Journal Article