Sensitivity of Dot-ELISA on Nitrocellulose Membranes in Comparison with ELISA on Polystyrene Plates for the Detection of Four Plant Viruses
Data(s) |
1987
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Resumo |
ABSTR.4CT Senitivity of dot-immunobindinding ELf SA on nitrocellulose membrane (DotELISA)was compared with double-antibody sandwich ELISA (DAS-ELlSA) on polystyrene plates for the detection of bean yellow mosaic virus (BYMV), broad bean stain virus (WMV-2). Dot-ELISA was 2 and 1O times more sensitive than DAS-ELISA for the detection of BBSV and WMV-2, respectively, whereas DAS-ELISA was more sensitive than Dot-ELiSA for {he detection of BYMV. Both techniques were equally sensitive for the detection of BYDV. Using one day instead uf the two-day procedure, the four viruses were still detectable and the ralative sensitivity of both techniques remained the same. |
Formato |
application/pdf |
Identificador | |
Publicador |
Lebanese National Council for Scientific Research |
Relação |
http://eprints.qut.edu.au/39789/1/Sensitivity_of_dot-ELISA_on_nictrocellulose_membranes_in_comparison_with_ELISA_medium.pdf http://www.cnrs.edu.lb/info/lsjIssues.html Makkouk, K.M., Jefferson, O., & Katul, L. (1987) Sensitivity of Dot-ELISA on Nitrocellulose Membranes in Comparison with ELISA on Polystyrene Plates for the Detection of Four Plant Viruses. Lebanese Science Bulletin, 3, pp. 29-35. |
Fonte |
Faculty of Science and Technology |
Palavras-Chave | #070600 HORTICULTURAL PRODUCTION #Sensitivity of dot-Elisa #Nitrocellulose Membranes #Polystyrene plates #Detection of four plant viruses #Jefferson, Osmat, Ossmat, Azzam |
Tipo |
Journal Article |