Mechanistic investigations into interactions between IGF-I and IGFBPs and their impact on facilitating cell migration on vitronectin


Autoria(s): Kricker, Jennifer A.; Hyde, Carolyn E.; Van Lonkhuyzen, Derek R.; Hollier, Brett G.; Shooter, Gary K.; Leavesley, David I.; Herington, Adrian C.; Upton, Zee
Data(s)

2010

Resumo

Numerous studies have reported links between insulin-like growth factors (IGFs) and the extra-cellular matrix protein vitronectin (VN). We ourselves have reported that IGF-I binds to VN via IGF-binding proteins (IGFBPs) to stimulate HaCaT and MCF-7 cell migration. Here, we detail the functional evaluation of IGFBP-1, -2, -3, -4 and -6 in the presence and absence of IGF-I and VN. The data presented here, combined with our prior data on IGFBP-5, suggest that IGFBP-3, -4 and -5 are the most effective at stimulating cell migration in combination with IGF-I and VN. In addition, we demonstrate that different regions within IGFBP-3 and -4 are critical for complex formation. Furthermore, we examine whether multi-protein complexes of IGF-I and IGFBPs associated with fibronectin and collagen IV are also able to enhance functional biological responses.

Identificador

http://eprints.qut.edu.au/39214/

Publicador

Informa Healthcare / Taylor & Francis

Relação

DOI:10.3109/08977194.2010.494603

Kricker, Jennifer A., Hyde, Carolyn E., Van Lonkhuyzen, Derek R., Hollier, Brett G., Shooter, Gary K., Leavesley, David I., Herington, Adrian C., & Upton, Zee (2010) Mechanistic investigations into interactions between IGF-I and IGFBPs and their impact on facilitating cell migration on vitronectin. Growth Factors, 28(5), pp. 359-369.

Direitos

Copyright 2010 Informa Healthcare/Taylor & Francis.

Fonte

Cell & Molecular Biosciences; Faculty of Science and Technology; Institute of Health and Biomedical Innovation; Science Research Centre

Palavras-Chave #060100 BIOCHEMISTRY AND CELL BIOLOGY #Vitronectin #IGF-I #IGFBP #cell migration #heparin-binding
Tipo

Journal Article