Migration of breast cancer cells : understanding the roles of volume exclusion and cell-to-cell adhesion


Autoria(s): Simpson, Matthew; Towne, Chris L.; McElwain, Sean; Upton, Zee
Data(s)

01/10/2010

Resumo

We study MCF-7 breast cancer cell movement in a transwell apparatus. Various experimental conditions lead to a variety of monotone and nonmonotone responses which are difficult to interpret. We anticipate that the experimental results could be caused by cell-to-cell adhesion or volume exclusion. Without any modeling, it is impossible to understand the relative roles played by these two mechanisms. A lattice-based exclusion process random-walk model incorporating agent-to-agent adhesion is applied to the experimental system. Our combined experimental and modeling approach shows that a low value of cell-to-cell adhesion strength provides the best explanation of the experimental data suggesting that volume exclusion plays a more important role than cell-to-cell adhesion. This combined experimental and modeling study gives insight into the cell-level details and design of transwell assays.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/38944/

Publicador

American Physical Society

Relação

http://eprints.qut.edu.au/38944/1/c38944a.pdf

DOI:10.1103/PhysRevE.82.041901

Simpson, Matthew, Towne, Chris L., McElwain, Sean, & Upton, Zee (2010) Migration of breast cancer cells : understanding the roles of volume exclusion and cell-to-cell adhesion. Physical Review E (PRE), 82(4), 041901-1---41901-9.

Direitos

Copyright 2010 The American Physical Society

Fonte

Faculty of Science and Technology; Institute of Health and Biomedical Innovation; Mathematical Sciences

Palavras-Chave #010202 Biological Mathematics #Breast Cancer #Exclusion process #Transwell #Adhesion
Tipo

Journal Article