Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model
Data(s) |
2006
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Resumo |
The osteogenic potential of human adipose-derived precursor cells seeded on medical-grade polycaprolactone-tricalcium phosphate scaffolds was investigated in this in vivo study. Three study groups were investigated: (1) induced—stimulated with osteogenic factors only after seeding into scaffold; (2) preinduced—induced for 2 weeks before seeding into scaffolds; and (3) uninduced—cells without any introduced induction. For all groups, scaffolds were implanted subcutaneously into the dorsum of athymic rats. The scaffold/cell constructs were harvested at the end of 6 or 12 weeks and analyzed for osteogenesis. Gross morphological examination using scanning electron microscopy indicated good integration of host tissue with scaffold/cell constructs and extensive tissue infiltration into the scaffold interior. Alizarin Red histology and immunostaining showed a heightened level of mineralization and an increase in osteonectin, osteopontin, and collagen type I protein expression in both the induced and preinduced groups compared with the uninduced groups. However, no significant differences were observed in these indicators when compared between the induced and preinduced groups. |
Identificador | |
Publicador |
Elsevier Ltd |
Relação |
DOI:10.1111/j.1432-0436.2006.00092.x Leong, David, Abraham, Mohan Chothirakottu, Rath, Subha Narayan, Lim, Thiam Chye, Chew, Fook Tim, & Hutmacher, Dietmar (2006) Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model. Differentiation, 74(9/10), pp. 519-529. |
Fonte |
Faculty of Built Environment and Engineering |
Palavras-Chave | #060100 BIOCHEMISTRY AND CELL BIOLOGY #069900 OTHER BIOLOGICAL SCIENCES #090300 BIOMEDICAL ENGINEERING #progenitor cells, osteo-differentiation, osteogenesis, animal model, bone tissue engineering |
Tipo |
Journal Article |