An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects


Autoria(s): Kolambkar, Yash M.; Dupont, Kenneth M.; Boerckel, Joel D.; Huebsch, Nathaniel; Mooney, David J.; Hutmacher, Dietmar W.; Guldberg, Robert E.
Data(s)

01/01/2011

Resumo

The treatment of challenging fractures and large osseous defects presents a formidable problem for orthopaedic surgeons. Tissue engineering/regenerative medicine approaches seek to solve this problem by delivering osteogenic signals within scaffolding biomaterials. In this study, we introduce a hybrid growth factor delivery system that consists of an electrospun nanofiber mesh tube for guiding bone regeneration combined with peptide-modified alginate hydrogel injected inside the tube for sustained growth factor release. We tested the ability of this system to deliver recombinant bone morphogenetic protein-2 (rhBMP-2) for the repair of critically-sized segmental bone defects in a rat model. Longitudinal [mu]-CT analysis and torsional testing provided quantitative assessment of bone regeneration. Our results indicate that the hybrid delivery system resulted in consistent bony bridging of the challenging bone defects. However, in the absence of rhBMP-2, the use of nanofiber mesh tube and alginate did not result in substantial bone formation. Perforations in the nanofiber mesh accelerated the rhBMP-2 mediated bone repair, and resulted in functional restoration of the regenerated bone. [mu]-CT based angiography indicated that perforations did not significantly affect the revascularization of defects, suggesting that some other interaction with the tissue surrounding the defect such as improved infiltration of osteoprogenitor cells contributed to the observed differences in repair. Overall, our results indicate that the hybrid alginate/nanofiber mesh system is a promising growth factor delivery strategy for the repair of challenging bone injuries.

Identificador

http://eprints.qut.edu.au/37829/

Publicador

Elsevier

Relação

DOI:10.1016/j.biomaterials.2010.08.074

Kolambkar, Yash M., Dupont, Kenneth M., Boerckel, Joel D. , Huebsch, Nathaniel, Mooney, David J. , Hutmacher, Dietmar W., & Guldberg, Robert E. (2011) An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects. Biomaterials, 32(1), pp. 65-74.

http://purl.org/au-research/grants/arc/DP0989000

Direitos

Copyright 2010 Elsevier

This is the author’s version of a work that was accepted for publication in Biomaterials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials, [VOL 32, ISSUE 1, (2011)] DOI: 10.1016/j.biomaterials.2010.08.074

Fonte

Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation; Science & Engineering Faculty

Palavras-Chave #090301 Biomaterials #Bone regeneration #Scaffold #Drug delivery #Bone morphogenetic protein #Nanofiber mesh #Alginate
Tipo

Journal Article