Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly (e-caprolactone)-tricalcium phosphate-collagen scaffolds


Autoria(s): Chum, Zhi Zhen; Woodruff, Maria A.; Cool, Simon M.; Hutmacher, Dietmar W.
Data(s)

2009

Resumo

We evaluate the potential of heparin as a substrate component for the fabrication of bone tissue engineering constructs using poly(e- caprolactone)–tricalcium phosphate–collagen type I (PCL–TCP–Col) three-dimensional (3-D) scaffolds. First we explored the ability of porcine bone marrow precursor cells (MPCs) to differentiate down both the adipogenic and osteogenic pathways within 2-D culture systems, with positive results confirmed by Oil-Red-O and Alizarin Red staining, respectively. Secondly, we examined the influence of heparin on the interaction and behaviour of MPCs when seeded onto PCL–TCP–Col 3-D scaffolds, followed by their induction into the osteogenic lineage. Our 3-D findings suggest that cell metabolism and proliferation increased between days 1 and 14, with deposition of extracellular matrix also observed up to 28 days. However, no noticeable difference could be detected in the extent of osteogenesis for PCL–TCP–Col scaffolds groups with the addition of heparin compared to identical control scaffolds without the addition of heparin.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/32409/

Publicador

Elsevier BV

Relação

http://eprints.qut.edu.au/32409/1/c32409.pdf

DOI:10.1016/j.actbio.2009.05.015

Chum, Zhi Zhen, Woodruff, Maria A., Cool, Simon M., & Hutmacher, Dietmar W. (2009) Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly (e-caprolactone)-tricalcium phosphate-collagen scaffolds. Acta Biomaterialia, 5(9), pp. 3305-3315.

Direitos

Copyright 2009 Elsevier BV

Fonte

Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation

Palavras-Chave #090301 Biomaterials #polycaprolactone #heparin #bone marrow #scaffold #differentiation
Tipo

Journal Article