Enhancing in vivo vascularized bone formation by cobalt chloride-treated bone marrow stromal cells in a tissue engineered periosteum model


Autoria(s): Fan, Wei; Crawford, Ross W.; Xiao, Yin
Data(s)

01/05/2010

Resumo

The periosteum plays an indispensable role in both bone formation and bone defect healing. In this study we constructed an artificial in vitro periosteum by incorporating osteogenic differentiated bone marrow stromal cells (BMSCs) and cobalt chloride (CoCl(2))-treated BMSCs. The engineered periostea were implanted both subcutaneously and into skull bone defects in SCID mice to investigate ectopic and orthotopic osteogenesis and vascularization. After two weeks in subcutaneous and four weeks in bone defect areas, the implanted constructs were assessed for ectopic and orthotopic osteogenesis and vascularization by micro-CT, histomorphometrical and immunohistochemical methods. The results showed that CoCl(2) pre-treated BMSCs induced higher degree of vascularization and enhanced osteogenesis within the implants in both ectopic and orthotopic areas. This study provided a novel approach using BMSCs sourced from the same patient for both osteogenic and pro-angiogenic purposes in constructing tissue engineered periosteum to enhance vascularized osteogenesis.

Formato

application/pdf

Identificador

http://eprints.qut.edu.au/31500/

Publicador

Elsevier BV

Relação

http://eprints.qut.edu.au/31500/1/c31500.pdf

DOI:10.1016/j.biomaterials.2010.01.083

Fan, Wei, Crawford, Ross W., & Xiao, Yin (2010) Enhancing in vivo vascularized bone formation by cobalt chloride-treated bone marrow stromal cells in a tissue engineered periosteum model. Biomaterials, 31(13), pp. 3580-3589.

Direitos

Copyright 2010 Elsevier BV

Fonte

Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation

Palavras-Chave #110300 CLINICAL SCIENCES #Periosteum #Tissue engineering #Osteoblasts #Vascularization #Cobalt Chloride
Tipo

Journal Article