Discrepancies between metabolic activity and DNA content as tool to assess cell proliferation in cancer research
Data(s) |
15/04/2010
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Resumo |
Cell proliferation is a critical and frequently studied feature of molecular biology in cancer research. Therefore, various assays are available using different strategies to measure cell proliferation. Metabolic assays such as AlamarBlue, WST-1, and MTT, which were originally developed to determine cell toxicity, are being used to assess cell numbers. Additionally, proliferative activity can be determined by quantification of DNA content using fluorophores, such as CyQuant and PicoGreen. Referring to data published in high ranking cancer journals, 945 publications applied these assays over the past 14 years to examine the proliferative behaviour of diverse cell types. Within this study, mainly metabolic assays were used to quantify changes in cell growth yet these assays may not accurately reflect cellular proliferation rates due to a miscorrelation of metabolic activity and cell number. Testing this hypothesis, we compared metabolic activity of different cell types, human cancer cells and primary cells, over a time period of 4 days using AlamarBlue and fluorometric assays CyQuant and PicoGreen to determine their DNA content. Our results show certain discrepancies in terms of over-estimation of cell proliferation with respect to the metabolic assay in comparison to DNA binding fluorophores. |
Formato |
application/pdf |
Identificador | |
Publicador |
Wiley-Blackwell Publishing Ltd. |
Relação |
http://eprints.qut.edu.au/31242/1/c31242.pdf DOI:10.1111/j.1582-4934.2010.01013.x Quent, Verena M.C., Loessner, Daniela L., Friis, Thor E., Reichert, Johannes C., & Hutmacher, Dietmar W. (2010) Discrepancies between metabolic activity and DNA content as tool to assess cell proliferation in cancer research. Journal of Cellular and Molecular Medicine, 14(4), pp. 1003-1013. |
Direitos |
Copyright 2010 The Authors Journal compilation Copyright 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
Fonte |
Faculty of Built Environment and Engineering; Faculty of Science and Technology; Institute of Health and Biomedical Innovation; School of Engineering Systems |
Palavras-Chave | #060103 Cell Development Proliferation and Death #cell proliferation #metabolic activity #proliferation assay #DNA content #cancer cells |
Tipo |
Journal Article |